The research object of transcriptome sequencing is the sum of all RNA that a specific cell can transcribe in a certain functional state, including mRNA and non coding RNA. Compared to traditional chip hybridization platforms, transcriptome sequencing can detect the overall transcriptional activity of any species without the need to pre design probes for known sequences, providing more accurate digital signals, higher detection throughput, and a wider detection range. It is currently a powerful tool for in-depth research on transcriptome complexity. Transcriptome sequencing technology based on high-throughput sequencing platforms can comprehensively obtain transcriptome information of specific tissues or organs of species, enabling research on gene expression levels, discovery of new transcripts, and structural variation of transcripts.
